Boric Acid Lactose Broth as a Medium for the Detection of Fecal Coliform Bacteria
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چکیده
منابع مشابه
Boric acid lactose broth as a medium for the detection of fecal coliform bacteria.
Boric acid lactose broth has been suggested (Levine et al., 1934; Vaughn and Levine, 1935; Vaughn et al., 1951; Levine et al., 1955) as a medium for the detection of Escherichia coli in water and in foods. This medium is designed to suppress Escherichia freundii and species of Aerobacter, organisms often considered to be nonfecal in origin. The published results are rather convincing where sepa...
متن کاملPossible explanations for the inhibitory action of boric acid lactose broth at 43 C on fecal coliform bacteria.
It has been shown (Njoku-Obi and Skinner, 1957) that the most probable number of coliforms and of Escherichia species in human feces is consistently higher when counts are made by dilution extinction methods in standard lactose broth at 35 C than when boric acid lactose broth (Vaughn et al., 1951) at 43 C is used. This is an example of a general phenomenon repeatedly noted in the literature. An...
متن کاملThe coliform group. I. The boric acid lactose broth reaction of coliform IMViC types.
Several methods have been suggested for the accurate, rapid, and convenient detection and enumeration of Escherichia coli in potable waters, stream samples, sewage, and in certain food products. The first of these procedures was described by Eijkman who proposed a "fecal coli" test that he believed differentiated between coliforms from the gut of warm-blooded animals and strains originating fro...
متن کاملA comparison of standard lactose broth with lauryl sulphate broth and with the Eijkman method for demonstrating fecal coliform bacteria.
Lauryl sulphate broth, introduced by Cowles (1938) and modified by Mallman and Darby (1941), has been widely used in recent years for the presumptive test for coliform bacteria in water. Lauryl sulphate is, to a certain extent, an inhibitory agent and tends to suppress anaerobic lactose fermenting bacteria. Inhibitory agents in culture media are always subject to suspicion. A substance which ca...
متن کاملComparison of fecal coliform agar and violet red bile lactose agar for fecal coliform enumeration in foods.
A 24-h direct plating method for fecal coliform enumeration with a resuscitation step (preincubation for 2 h at 37 +/- 1 degrees C and transfer to 44 +/- 1 degrees C for 22 h) using fecal coliform agar (FCA) was compared with the 24-h standardized violet red bile lactose agar (VRBL) method. FCA and VRBL have equivalent specificities and sensitivities, except for lactose-positive non-fecal colif...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1957
ISSN: 0003-6919
DOI: 10.1128/am.5.2.80-82.1957